किपा:Summary of SAGE.svg
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Summary
| Description |
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
| Date | |
| Source | Own work |
| Author | Thomas Shafee |
| Other versions |
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- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
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| Date/Time | Thumbnail | Dimensions | छ्य्लामि | Comment | |
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| current | १२:२१, १३ फेब्रुवरी २०१७ | | ९३८ × ७५६ (१८० KB) | Evolution and evolvability | text as paths to avoid artefacts |
| १०:४८, १३ फेब्रुवरी २०१७ |  | ९३८ × ७५६ (१०४ KB) | Evolution and evolvability | update sequence text to 'sans-serif' open font for more robust rendering | |
| ०५:२७, ७ अक्टोबर २०१६ |  | ९३८ × ७५६ (१०४ KB) | Evolution and evolvability | ce | |
| ०५:२३, ७ अक्टोबर २०१६ |  | ९३८ × ७५६ (१०२ KB) | Evolution and evolvability | ce | |
| ०५:१६, ७ अक्टोबर २०१६ |  | ९३८ × ७५६ (१०४ KB) | Evolution and evolvability | digitag-->tag | |
| ०५:०८, ५ अक्टोबर २०१६ |  | १,०२० × ८२१ (१०६ KB) | Evolution and evolvability | User created page with UploadWizard |
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